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Sequence organization and putative regulatory elements in the 5S rRNA genes of two higher plants (Vigna radiata and Matthiola incana)

1988; Elsevier BV; Volume: 62; Issue: 1 Linguagem: Inglês

10.1016/0378-1119(88)90591-4

1879-0038

Vera Hemleben, Dagmar Werts,

Genomics and Phylogenetic Studies

The tandemly arranged and clustered highly repeated 5S rRNA genes are investigated for two plants belonging to different higher plant families: Matthiola incana (Brassicaceae, Dilleniidae, Rosidae; 3600 5S rRNA genes/n) shows a homogeneous repeat size of 510bp, whereas Vigna radiata (mung bean, former Phaseolus aureus, Fabaceae, Rosidae; approx. 4300 5S rRNA genes) has a repeat size of 215 bp. The mung-bean 5S rRNA coding region starts 5' with AGG and ends with CCT; Matthiola starts with GGG and ends with CCC. Striking is the strict occurrence of a ‘TATA’ box starting at nucleotide −28 similar to Neurospora crassa 5S rRNA genes. The 3' end is followed by CTTTT or GTTTT stretches present in different numbers in the non-transcribed spacer suggesting a function in termination.