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Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy

2011; American Chemical Society; Volume: 133; Issue: 16 Linguagem: Inglês

10.1021/ja108404p

1943-2984

Hiroyuki Yamakoshi, Kosuke Dodo, Masaya Okada, Jun Ando, Almar F. Palonpon, Katsumasa Fujita, Satoshi Kawata, Mikiko Sodeoka,

RNA Interference and Gene Delivery

Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.