A new platelet-specific alloantigen system, Yuka/Yukb is located on platelet membrane glycoprotein IIIa.
1987; Japan Academy; Volume: 63; Issue: 1 Linguagem: Inglês
10.2183/pjab.63.36
ISSN1349-2896
Autores Tópico(s)Blood disorders and treatments
ResumoThe development of alloantibodies to platelet-specific antigens following blood transfusion and during pregnancy causes post-transfusion purpura (PTP) and neonatal alloimmune thrombocytopenia (NAITP), respectively.In Caucasian, more than 90% of the reported cases of PTP') and NAITP2) are caused by antibodies to the Zwa antigen, one of the platelet-specific antigens.However, the frequency of Zwa negative individuals is very low in the Japanese population and so anti-Zwa appears to be a rare cause of PTP and NAITP in Japan.3)Recently, we found a new platelet-specific alloantigen system, Yuka/Yukb (allelic, inherited as autosomal codominant traits) involved in three cases of NAITP.3),4)The Yuka/Yukb antigens appear to play an important role in many cases of PTP and NAITP in Japan.The Yuka/Yukb antigens are not expressed on platelets from two patients with Glanzmann's thrombasthenia, suggesting that these antigens are present on platelet glycoprotein (GP) IIb and/or IIIa.41We now report that the Yuka /Yukb antigens reside on GP l:IIa.Material and method.Antisera : Anti-Yuka serum (S.Y.) and anti-Yukb serum (Y.O.) were from the mothers with children suffering from NAITP.3),4)Anti-Zwa serum was kindly supplied by Dr. Mueller-Eckhardt (Giessen, FRG).Iodination o f platelets:Platelets (Yuka+, Yukb+, Zwa+) were obtained by differential centrifugation of freshly drawn whole blood anticoagulated with CPD.These platelets were iodinated with 0.8 mCi carrier-free Na125I (New England Nuclear) by the method of Vitetta et al.)Triton X-100-solubilized platelet membrane preparation: 123I-labeled platelets were homogenized and sonicated in 20 mM Tris-HC1, pH 7.6, 150 mM NaCI, 2.5 mM EDTA, 1 mM PMSF.After centrifugation at 100,000xg for 30 min, the pellet was suspended in 0.3 mM sodium phosphate, pH 7.4, 2.5 mM EDTA followed by incubation at 37°C for 30 min.The suspension was again centrifuged and the resultant pellet was homogenized with 20 mM Tris-HC1, pH 7.6, 150 mM NaCI, 2.5 mM EDTA, 1 mM PMSF, 1 % Triton X-100.The homogenate was centrifuged and the supernatant was used as platelet membrane proteins.Immunoprecipitation : Six microliters (1.0x106 cpm) of solubilized platelet membrane proteins were incubated at 37°C for 60 min with 12 J of each of the following: control human sera, anti-Yuka, anti-Yukb and anti-Zwa in TSG buffer (50 mM Tris-HC1, pH 7.6, 150 mM NaCI, 1 % goat serum).Fifteen microliters
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