Human Responses to Influenza Vaccination Show Seroconversion Signatures and Convergent Antibody Rearrangements
2014; Cell Press; Volume: 16; Issue: 1 Linguagem: Inglês
10.1016/j.chom.2014.05.013
ISSN1934-6069
AutoresKatherine Jackson, Yi Liu, Krishna M. Roskin, Jacob Glanville, Ramona A. Hoh, Katie Seo, Eleanor L. Marshall, Thaddeus C. Gurley, M. Anthony Moody, Barton F. Haynes, Emmanuel B. Walter, Hua‐Xin Liao, Randy A. Albrecht, Adolfo Garcı́a-Sastre, Javier Chaparro‐Riggers, Arvind Rajpal, Jaume Pons, Birgitte B. Simen, Bozena Hanczaruk, Cornelia L. Dekker, Jonathan Laserson, Daphne Koller, Mark M. Davis, Andrew Fire, Scott D. Boyd,
Tópico(s)HIV Research and Treatment
ResumoHighlights•Human antibody gene repertoire sequencing identifies vaccine-specific B cell clones•Quantified B cell clonal expansions correlate with serological vaccine responses•Human pandemic H1N1 2009 influenza vaccine responses show convergent antibodiesSummaryB cells produce a diverse antibody repertoire by undergoing gene rearrangements. Pathogen exposure induces the clonal expansion of B cells expressing antibodies that can bind the infectious agent. To assess human B cell responses to trivalent seasonal influenza and monovalent pandemic H1N1 vaccination, we sequenced gene rearrangements encoding the immunoglobulin heavy chain, a major determinant of epitope recognition. The magnitude of B cell clonal expansions correlates with an individual's secreted antibody response to the vaccine, and the expanded clones are enriched with those expressing influenza-specific monoclonal antibodies. Additionally, B cell responses to pandemic influenza H1N1 vaccination and infection in different people show a prominent family of convergent antibody heavy chain gene rearrangements specific to influenza antigens. These results indicate that microbes can induce specific signatures of immunoglobulin gene rearrangements and that pathogen exposure can potentially be assessed from B cell repertoires.Graphical abstract
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