Factors influencing the determination of cellular morphology in cells infected with Rous sarcoma virus
1962; Elsevier BV; Volume: 18; Issue: 4 Linguagem: Inglês
10.1016/0042-6822(62)90053-3
ISSN1096-0341
Autores Tópico(s)Virology and Viral Diseases
ResumoAdditional means have been described for controlling the presence or absence, and nature, of morphological transformation induced by Rous sarcoma virus infection of turkey fibroblasts in vitro. Eagle's medium supplemented with serum from various species was found to support only limited morphological transformation under normal circumstances. The addition of 10% tryptose broth to such media markedly favored the production of morphological transformation. Infection of cells at various times after a 24-hour period of starvation in serum-free Eagle's solution without glucose and glutamine at room temperature resulted in marked variations in both the total extent of morphological transformation and the nature of the transformation which was observed. Tumors induced in vivo by single virus particles varied widely in morphology. Tumors induced in this manner having predominantly spindle-cell, giant-cell, fusiformcell, and round-cell morphologies were observed. The above findings are discussed particularly in reference to possible correlations between in vitro and in vivo morphology, and in vitro morphological conversion with neoplastic potential; and possible mechanisms underlying the production of morphologic conversion by Rous sarcoma virus.
Referência(s)