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Regulation of Membrane Properties of Mitochondria and Plastids During Chloroplast Development I. The Action of Phytochrome in situ

1977; Elsevier BV; Volume: 82; Issue: 1 Linguagem: Inglês

10.1016/s0044-328x(77)80134-7

0044-328X

Rüdiger Hampp, H.W. Schmidt,

Algal biology and biofuel production

Summary Mitochondria and plastids were isolated from etiolated laminae of Avena sativa L. given a special light program (different red and far-red light treatments). Both types of organelles exhibited the same changes in the penetrability * of their transport limiting membranes described earlier for white light induced plastid development. Continuous irradiation with far-red light increased the penetrability significantly up to 3 h of light treatment; there-after a decrease in the internal concentration of the 14C-labelled compounds tested (δ-aminolevulinate, succinate) was observed. The same effects were induced after a 5 min treatment with red light followed by darkness. Additionally, induction-reversion experiments performed by irradiating the etiolated seedlings with far-red light (5 min), immediately following the red light irradiations, demonstrated that the effect of red light could be reversed for both mitochondria and etioplasts. Hence, the operational criterion for the involvement of phytochrome is fulfilled. The results therefore indicate that the changes of membrane penetrability observed are mediated by phytochrome.