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Preparation of B Cell Deficient Guinea Pig Islets of Langerhans

1971; Thieme Medical Publishers (Germany); Volume: 3; Issue: 01 Linguagem: Inglês

10.1055/s-0028-1095045

1439-4286

Simon L. Howell, John C. Edwards, M. Whitfield,

Adenosine and Purinergic Signaling

Islets of Langerhans were isolated from guinea pig pancreas six days after the animals had received an intraperitoneal injection of streptozotocin. These islets were depleted of at least 90% of their insulin content and of B cells as judged by immunoassay and by electron microscopy, while the ultrastructure of the other cell type remained entirely normal The secretory response of the A cells, which constituted about 70% of the cell population of the B cell deficient islets, were very similar to those of A cells in islets of Langerhans isolated from normal guinea pig. This preparation may provide the basis for a method of separating a population consisting solely of the glucagon-producing cell type.