Do β-tubulin pseudogenes really matter?
2004; Elsevier BV; Volume: 5; Issue: 5 Linguagem: Inglês
10.1016/s1470-2045(04)01464-0
ISSN1474-5488
AutoresCristóbal Belda-Iniesta, Rosario Perona, Javier de Castro, Sharmila Chattopadhyay, E. Casado, Paloma Cejas, Juan Ángel Fresno Vara, Concepcion Moreno Guerra, Manuel González Barón,
Tópico(s)Lung Cancer Treatments and Mutations
ResumoWe read with interest the review on β-tubulin mutations and resistance to chemotherapy by Berrieman and colleagues in the March issue of The Lancet Oncology. Several considerations about β-tubulin pseudogenes, however, must be taken into account. First, although at least nine β-tubulin pseudogenes have been described, careful in silico analysis has shown that several GenBank assignations have the same, unique sequence. For example, accessions V00598, J00317, K00841, K00840, and M24191 are named HSTUB1, HUMTBB46P, HUMTBB21P, HUMTBB14P, and HUMTBB46QP, respectively. All these sequences correspond to the same ENSEMBL accession number ENSESTG00000019284, which is located at 8p12. This sequence, identifiable with exon primers designed by Monzo and colleagues,2 includes the conflicting region noted above that has many designations. However, most mutations in this particular pseudogene are in fact changes to the “normal DNA sequence”. 3 de Castro J Belda-Iniesta C Cejas P et al. New insights in beta-tubulin sequence analysis in non-small cell lung cancer. Lung Cancer. 2003; 41: 41-48 Summary Full Text Full Text PDF PubMed Scopus (16) Google Scholar In our opinion, to avoid confusion in future studies on β-tubulin pseudogenes, this consideration is very important.
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