Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples
2001; Elsevier BV; Volume: 442; Issue: 1 Linguagem: Inglês
10.1016/s0003-2670(01)01137-0
ISSN1873-4324
AutoresMark P. Kreuzer, Ciara K. O’Sullivan, Martin Pravda, G. G. Guilbault,
Tópico(s)Molecular Junctions and Nanostructures
ResumoThe current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml−1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.
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