Further studies on the formation of oxygen radicals by potassium superoxide in aqueous medium for biochemical investigations
1986; Elsevier BV; Volume: 34; Issue: 1 Linguagem: Inglês
10.1016/0378-4274(86)90147-5
ISSN1879-3169
AutoresBelur R. Lokesh, Michael L. Cunningham,
Tópico(s)Mitochondrial Function and Pathology
ResumoPotassium Superoxide (KO2) forms Superoxide anion (O2−) in aqueous medium as measured by the Superoxide dismutase (SOD)-inhibitable cytochrome c reduction assay of McCord and Fridovich [1]. The reduction of cytochrome c by O2− formed by KO2 was observed only above pH 7.0 and demonstrated a pH optimum at pH 9.6. SOD was an effective inhibitor of the cytotochrome c reduction produced by KO2. Hydroxyl radical scavengers and singlet oxygen quenchers did not interfere with the reduction of cytochrome c by KO2. These data demonstrate that addition of KO2 to aqueous medium is an easy chemical method for the production of O2− in controlled amounts.
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