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Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

1989; National Academy of Sciences; Volume: 86; Issue: 2 Linguagem: Inglês

10.1073/pnas.86.2.577

1091-6490

Karen M. Hahnenberger, Mary Baum, Catherine M. Polizzi, John Carbon, Ian N. Clarke,

Chromosomal and Genetic Variations

The centromere DNAs from chromosomes I and III of Schizosaccharomyces pombe have been cloned in an artificial chromosome vector in both budding and fission yeasts. In S. pombe, synthetic linear and circular minichromosomes containing an intact centromere are stable mitotically and behave as independent genetic linkage groups that segregate properly through meiosis. These experiments present a general strategy for the isolation of centromeres from other organisms.