Portal de Periódicos da CAPES

Evolution of P transposable elements: sequences of Drosophila nebulosa P elements.

1987; National Academy of Sciences; Volume: 84; Issue: 18 Linguagem: Inglês

10.1073/pnas.84.18.6491

1091-6490

Robert A. Lansman, Rosemary O. Shade, T A Grigliatti, Hugh W. Brock,

RNA Research and Splicing

P elements have been cloned and sequenced from Drosophila nebulosa. Their sequences have diverged less than 6% from P elements of Drosophila melanogaster. However D. nebulosa P elements have nucleotide changes that close all four open reading frames found in the D. melanogaster P element. Microinjection experiments show that D. nebulosa P elements cannot provide transposase function for D. melanogaster P elements, nor are D. nebulosa P elements mobilized by the transposase provided by a D. melanogaster P factor. Three D. nebulosa P elements appear to have integrated into the same position of a complex, centromeric repeated sequence. Comparison of nucleotide sequences suggests that D. nebulosa P elements have diverged upon different pathways from a common ancestor that was 99% homologous to the P elements of D. melanogaster.