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In vitro DNA binding studies of anticancer drug idarubicin using spectroscopic techniques

2014; Elsevier BV; Volume: 138; Linguagem: Inglês

10.1016/j.jphotobiol.2014.05.015

1873-2682

Can Ozluer, Hayriye Eda Şatana Kara,

Protein Interaction Studies and Fluorescence Analysis

The interaction between idarubicin and double stranded deoxyribonucleic acid (ds-DNA) was investigated by UV–VIS spectrophotometry, fluorescence and Raman spectroscopy techniques. The absorption spectra of idarubicin with ds-DNA showed a slight red shift and hypochromic effect. In the fluorescence experiments, emission peaks were decreased by adding ds-DNA. Using ethidium bromide (ETB) as a fluorescence probe, fluorescence quenching of the emission peak was observed in the ETB-DNA system when idarubicin was added. Moreover, similar results were obtained in Raman spectroscopy. Binding constants of idarubicin with ds-DNA were determined as 5.14 × 105 M−1 and 5.8 × 105 M−1 for UV–VIS spectrophotometry and fluorescence spectroscopy, respectively. The large binding constant indicated that idarubicin has a high affinity with ds-DNA. All the evidences indicated that the binding mode of idarubicin with DNA was an intercalative binding. Furthermore, quantitative determination of idarubicin in pharmaceutical formulation was done.