Electrochemical reduction and determination of cimetidine at nanomolar to micromolar levels of concentration
1983; Elsevier BV; Volume: 154; Linguagem: Inglês
10.1016/0003-2670(83)80011-7
ISSN1873-4324
AutoresAndrew Webber, Mumtaz H. Shah, Janet Osteryoung,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoThe electrochemical reduction of cimetidine, an H2-antagonist of histamine used in the treatment of duodenal and gastric ulcers, has been examined by using a variety of electrochemical techniques. Reduction in 0.1. M HCl occurs at −0.8 to −1.1 V vs. SCE depending on the experimental conditions. Square-wave and cyclic voltammetry were found to be the best techniques for the determination of cimetidine whereas sampled-d.c., normal pulse, and differential pulse polarography were not useful. The detection limit for the determination depends on the condition employed but can be as low as 1 ng ml−1 (4 nM). A linear calibration plot is obtained up to 2 μg ml−1 and calibration curves can be used at higher concentrations. The high sensitivity is due to reactant adsorption. The electrode process appears to involve a 4-electron reduction of the cyano group in cimetidine. The major metabolites of cimetidine can also be determined by this method.
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