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Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein

1992; Elsevier BV; Volume: 118; Issue: 2 Linguagem: Inglês

10.1016/0378-1119(92)90198-x

1879-0038

Mathieu H. M. Noteborn, Onno Kranenburg, A. Zantema, Guus Koch, G. F. de Boer, Alex J. van der Eb,

Viral gastroenteritis research and epidemiology

This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al. J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its transcription start point and poly(A)-addition site are located at nt 354 and 2317 of the CAV sequence, respectively. In vitro translation experiments provide evidence that the major CAV open reading frame encodes a 52-kDa protein by using the fifth AUG as a start codon of the unspliced CAV mRNA.