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Gas chromatographic/mass spectrometric analysis of high-performance liquid chromatographic fractions reflecting arachidonic acid metabolism in mouse peritoneal macrophages

1992; Wiley; Volume: 21; Issue: 2 Linguagem: Inglês

10.1002/bms.1200210203

2376-3876

Joaquín Abián, G. Bioque, O. Bulbena, J Roselló, Ellen Gelpí,

Analytical Chemistry and Chromatography

Biological Mass SpectrometryVolume 21, Issue 2 p. 69-79 Article Gas chromatographic/mass spectrometric analysis of high-performance liquid chromatographic fractions reflecting arachidonic acid metabolism in mouse peritoneal macrophages J. Abián, J. Abián Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorG. Bioque, G. Bioque Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorO. Bulbena, O. Bulbena Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorJ. Roselló, J. Roselló Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorE. Gelpí, Corresponding Author E. Gelpí Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainDepartment of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this author J. Abián, J. Abián Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorG. Bioque, G. Bioque Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorO. Bulbena, O. Bulbena Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorJ. Roselló, J. Roselló Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this authorE. Gelpí, Corresponding Author E. Gelpí Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainDepartment of Neurochemistry (Eicosanoid Branch), CID-CSIC, J. Girona 18–26, 08034 Barcelona, SpainSearch for more papers by this author First published: February 1992 https://doi.org/10.1002/bms.1200210203Citations: 9AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Mouse peritoneal macrophages are used as a model for studies undertaken around the oxidative metabolism of arachidonic acid elicted by xenobiotics (N-phenyllinoleamide, related to the toxic oil syndrome, has been used as an example). A high-performance liquid chromatographic method for cyclo- and lipoxygenase metabolite fractionation has been developed. Gas chromatographic/mass spectrometric analysis of the high-performance liquid chromato-graphic fractions thus obtained show that the major products detected in the incubates correspond to three principal structures: monohydroxy acids (12-hydroxyeicosatetraenoic acid being the major component), epoxyhydroxy acids and trihydroxy acids. Other minor compounds such as 12-hydroxyheptadecatrienoic acid, various dihydroxy acids and prostaglandins were also detected. Cells pre-exposed to N-phenyllinoleamide show selectively enhanced levels of 6-keto prostaglandin F1α, as measured by both gas chromatography/mass spectrometry and radioimmunoassay of the corresponding high-performance liquid chromatographic fraction. References 1 WHO Summary Report of the TOS working group held in Madrid, 21–24 March 1983, Nature 302, 737 (1983). 2 A. Pestaña and E. Muñoz, Nature 298, 608 (1982). 3 E. M. Kilbourne et al. And the Toxico-Epidemiologic Study Group, Am. J. Epidemiol. 127, 1210 (1988). 4 I. Ramis, G. Bioque, J. Roselló, E. Feliu, O. Bulbena and E. Gelpi, Prost. Leuk. Essent. Fatty Acids 39, 147 (1990). 5 E. Gelpí, I. Ramis, G. Hotter, G. Bioque, O. Bulbena and J. Roselló, J. Chromatogr. Biomed. Appl. 492, 223 (1989). 6 S. W. Chensue, D. A. Ellul, M. Spengler, G. I. Higashi and S. L. Kunkel, J. Leuk. Biol. 38, 671 (1985). 7 E. Gelpí and J. Abián, LC-GC Int. 3, 30 (1990). 8 M. Claeys, Mass Spectrom. Rev. 8, 1 (1989). 9 C. R. 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