Regeneration of Isolated Protoplasts of Vicia faba L.
1978; Elsevier BV; Volume: 88; Issue: 4 Linguagem: Inglês
10.1016/s0044-328x(78)80134-2
ISSN0044-328X
AutoresHorst Binding, Reinhard Nehls,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoProtoplasts of Vicia faba L. were isolated from tissue of the shoot apex and from leaves of various ages. They regenerated to calluses in media KM (Kao and Michayluk, 1975) and V-KM, composed of the inorganic salts of V-47 (Binding, 1974 a) and the organic ingredients of KM. Important factors were low titres of the Vicia protoplasts and temperatures of 22-26°C. Highest plating efficiencies were achieved if less than 3 · 103 protoplasts per ml of Vicia faba were co-cultured with 0.5-1.0 · 104 protoplasts of Petunia hybrida. The propagation of regenerated calluses was possible on media MS (Murashige and Skoog, 1962), NN (Nitsch and Nitsch, 1969) and NT (Nagata and Takebe, 1971) if the concentrations of cytokinins did not exceed 1µM.
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