Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation
2003; Oxford University Press; Volume: 221; Issue: 2 Linguagem: Inglês
10.1016/s0378-1097(03)00205-2
ISSN1574-6968
AutoresJean‐Luc Legras, Francis Karst,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoA new primer pair (delta12–delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.
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