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ACTIVATION OF THE ROSTRAL PONTINE RETICULAR FORMATION INCREASES THE SPINAL GLYCINE LEVEL AND INHIBITS BLADDER CONTRACTION IN RATS

2005; Lippincott Williams & Wilkins; Volume: 173; Issue: 5 Linguagem: Inglês

10.1097/01.ju.0000154646.11570.65

1527-3792

Saori Nishijima, Kimio Sugaya, Minoru Miyazato, Shuichi Shimabukuro, Makoto Morozumi, Yoshihide Ogawa,

Neuroscience of respiration and sleep

No AccessJournal of UrologyInvestigative Urology1 May 2005ACTIVATION OF THE ROSTRAL PONTINE RETICULAR FORMATION INCREASES THE SPINAL GLYCINE LEVEL AND INHIBITS BLADDER CONTRACTION IN RATS SAORI NISHIJIMA, KIMIO SUGAYA, MINORU MIYAZATO, SHUICHI SHIMABUKURO, MAKOTO MOROZUMI, and YOSHIHIDE OGAWA SAORI NISHIJIMASAORI NISHIJIMA More articles by this author , KIMIO SUGAYAKIMIO SUGAYA More articles by this author , MINORU MIYAZATOMINORU MIYAZATO More articles by this author , SHUICHI SHIMABUKUROSHUICHI SHIMABUKURO More articles by this author , MAKOTO MOROZUMIMAKOTO MOROZUMI More articles by this author , and YOSHIHIDE OGAWAYOSHIHIDE OGAWA More articles by this author View All Author Informationhttps://doi.org/10.1097/01.ju.0000154646.11570.65AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We examined the mechanism involved in the inhibition of bladder activity in rats by stimulating the rostral pontine reticular formation (RPRF) using carbachol, flavoxate and propiverine, and by analysis of amino acid levels in the lumbosacral cord. Materials and Methods: A total of 82 female rats were anesthetized with urethane. Under isovolumetric conditions physiological saline, carbachol, flavoxate or propiverine was injected into the RPRF or intravenously. Changes in bladder activity and amino acid levels in the lumbosacral cord were examined. Results: Injection of carbachol or flavoxate (0.3 μM each) into the RPRF abolished bladder contraction but there was no change after injection of physiological saline or propiverine. Intravenous injection of flavoxate or propiverine (0.1 to 10 mg/kg each) inhibited bladder contraction. Amino acid analysis revealed that injection of carbachol into the RPRF increased glutamate and glycine levels in the lumbosacral cord, while injection of flavoxate into the RPRF or intravenously caused an increase in glycine the lumbosacral cord. Injection of propiverine into the RPRF or intravenously did not influence lumbosacral cord amino acid levels. Conclusions: These results suggest that the RPRF has an important role in the inhibition of bladder contraction and carbachol or flavoxate can activate descending RPRF neurons and inhibit bladder contraction via spinal glycinergic neurons. References 1 : Pharmacology of the lower urinary tract. Annu Rev Pharmacol Toxicol2001; 41: 691. Google Scholar 2 : The physiology of excitatory amino acids in the vertebrate central nervous system. Prog Neurobiol1987; 28: 197. Google Scholar 3 : Basic neurophysiology and neuropharmacology. In: Incontinence, 1st International Consultation on Incontinence, June 28-July 1, 1998, Monaco. Edited by . Plymouth, United Kingdom: Health Publication, Ltd.1999: 105. Google Scholar 4 : Neurophysiology of micturition and its modification in animal models of human disease. In: . Edited by . London: Harwood Academic Publishers1993: 227. Google Scholar 5 : Inhibitory effect of intrathecal glycine on the micturition reflex in normal and spinal cord injury rats. 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Presented at the International Continence Society 33rd Annual Meeting, 2003 Google Scholar From the Division of Urology, Department of Organ-Oriented Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan© 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySugaya K, Nishijima S, Kadekawa K, Ashitomi K, Ueda T and Yamamoto H (2018) Intravenous or Local Injections of Flavoxate in the Rostral Pontine Reticular Formation Inhibit Urinary Frequency Induced by Activation of Medial Frontal Lobe Neurons in RatsJournal of Urology, VOL. 192, NO. 4, (1278-1285), Online publication date: 1-Oct-2014.Nishijima S, Sugaya K, Kadekawa K, Ashitomi K and Yamamoto H (2018) Effect of Chemical Stimulation of the Medial Frontal Lobe on the Micturition Reflex in RatsJournal of Urology, VOL. 187, NO. 3, (1116-1120), Online publication date: 1-Mar-2012.Nishijima S, Sugaya K, Miyazato M and Ogawa Y (2018) Effect of Gosha-Jinki-Gan, a Blended Herbal Medicine, on Bladder Activity in RatsJournal of Urology, VOL. 177, NO. 2, (762-765), Online publication date: 1-Feb-2007. Volume 173Issue 5May 2005Page: 1812-1816 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.Keywordsbladderreflexglycinespinal cordbrainMetricsAuthor Information SAORI NISHIJIMA More articles by this author KIMIO SUGAYA More articles by this author MINORU MIYAZATO More articles by this author SHUICHI SHIMABUKURO More articles by this author MAKOTO MOROZUMI More articles by this author YOSHIHIDE OGAWA More articles by this author Expand All Advertisement PDF downloadLoading ...