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Application of Sakaguchi reaction to the quantitative estimation of arginine: a method involving paper chromatography

1959; Elsevier BV; Volume: 84; Issue: 2 Linguagem: Inglês

10.1016/0003-9861(59)90600-9

1096-0384

K. R. Bhattacharya, Jyotirindra Datta, Durlav K. Roy,

RNA and protein synthesis mechanisms

Abstract In the Sakaguchi reaction of arginine, serious interference is caused by histidine, cystine, methionine, tryptophan, asparagine, and glutamine. The mechanism as well as the consequence of these interferences has been discussed. Suitable solvents to separate arginine from these amino acids by paper chromatography have also been indicated. The presence of a few other substances in a solution of arginine hydrochloride resulted in slightly higher color readings being given by unchromatographed spots of such solutions. This could be correlated with an apparent adsorption of arginine hydrochloride on filter paper, and its prevention by the presence of ions. Intensity as well as shade of the eluted color is dependent on pH. A method has been developed, involving paper chromatography, by which arginine can be reliably estimated in biological materials. Besides others, the method is also unaffected by the presence of ammonia. The arginine contents of some purified proteins have been determined by the present method.