Produção Nacional
Revisado por pares
An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection
2013; Brazilian Society of Tropical Medicine; Volume: 46; Issue: 6 Linguagem: Inglês
10.1590/0037-8682-1625-2013
ISSN1678-9849
AutoresAmanda de Fáveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fábio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson José Venâncio,
Tópico(s)Cytomegalovirus and herpesvirus research
ResumoIntroduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. Conclusions The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P. brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.
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