High-Performance Liquid Chromatographic Analysis of Methadone Hydrochloride Oral Solution
1977; Elsevier BV; Volume: 66; Issue: 12 Linguagem: Inglês
10.1002/jps.2600661224
ISSN1520-6017
AutoresThomas H. Beasley, Howard W. Ziegler,
Tópico(s)Analytical Methods in Pharmaceuticals
ResumoA direct and rapid high-performance liquid chromatographic assay for methadone hydrochloride in a flavored oral solution dosage form is described. A syrup sample, one part diluted with three parts of water, is introduced onto a column packed with octadecylsilane bonded on 10-μm porous silica gel (reversed phase). A formic acid-ammonium formate-buffered mobile phase is linear programmed with acetonitrile. The absorbance is monitored continuously at 280 or 254 nm, using a flowthrough, UV, double-beam photometer. An aqueous methadone hydrochloride solution is used for external standardization. The relative standard deviation was not more than 1.0%. Drug recovery from a syrup base was better than 99.8%.
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