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Gas chromatographic analysis of the higher fatty acids of the alga chlorella vulgaris (pyrenoidosa)

1972; Elsevier BV; Volume: 65; Issue: 2 Linguagem: Inglês

10.1016/s0021-9673(00)92559-7

1873-3778

M. Matucha, Luděk Žilka, K. Švihel,

Marine and coastal ecosystems

Abstract Fatty acids of the alga Chlorella vulgaris ( pyrenoidosa ) were analyzed by the gas-liquid chromatography of their methyl esters. By use of two (polar and non-polar) stationary phases, comparison with standards and bromination C 14 -C 20 fatty acids were detected and identified. In addition to the major acids (palmitic, oleic, linoleic and linolenic) the alga lipids contain n -14:0, 14:1, 14:2, 14:3, n -15:o, ai -15:o, 16:1, 16:2, 16:3, n -17:0, ai -17:0, n -18:0, n -19:0 and n -20:0 fatty acids at levels in excess of 0.1%. The results obtained are of importance in the analytical control of the radiochemical purity of 14 C-labelled higher fatty acids prepared from the alga. The presence of odd (straight-chain and branched) acids is interesting from biochemical viewpoint.