Diverse BF recombinants have spread widely since the introduction of HIV-1 into South America
2001; Lippincott Williams & Wilkins; Volume: 15; Issue: 15 Linguagem: Inglês
10.1097/00002030-200110190-00002
ISSN1473-5571
AutoresJean K. Carr, María Mercedes Ávila, Manuel Gómez Carrillo, Horacio Salomón, Jesse Hierholzer, Veerachai Watanaveeradej, María A. Pando, Mónica Negrete, Kevin L. Russell, José L. Sánchez, Deborah L. Birx, Ronald Andrade, José Viñoles, Francine E. McCutchan,
Tópico(s)Immunodeficiency and Autoimmune Disorders
ResumoObjective To describe the genetic diversity of HIV-1 in South America by full genome sequencing and analysis. Methods Purified peripheral blood mononuclear cell DNA from HIV-infected individuals in Argentina, Uruguay and Bolivia was used to amplify full HIV-1 genomes. These were sequenced using the ABI 3100 automated sequencer and phylogenetically analysed. Results Twenty-one HIV-1 strains from three South American countries, 17 of which were pre-screened by envelope heteroduplex mobility assay (HMA), were studied. Ten out of 10 HMA subtype F and four out of seven HMA subtype B strains were actually BF recombinants upon full genome analysis. Two BF recombinants from Argentina and two from Uruguay had the same structure, representing a new circulating recombinant form termed CRF12_BFARMA159. Twelve other BF recombinants had structures related to CRF12 but with additional segments of subtype B; each was unique. BF recombinants were temporally and geographically widespread, found as early as 1986–1987 in vertically infected Argentinian children and in Argentina, Uruguay, and Bolivia.
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