Ribosome Synthesis Compared During Pollen and Pollen Tube Development
1971; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-408-70149-5.50028-2
Autores Tópico(s)Plant Molecular Biology Research
ResumoThis chapter discusses the comparative study of ribosome synthesis, dealing with the other two components, the ribosomal proteins and 5S RNA. Microspores in detached buds were labeled in vitro with 14CO2 at the peak of ribosome synthesis. By contrast, if pollen tubes were labeled with 35S and the ribosomes were extracted, the resulting ribosomal proteins are not radioactive at all nor are the histones. The pollen tube offers a unique system to study different RNA molecules because the noise from ribosome synthesis is shut off. The most interesting finding about tRNA concerns its methylation in the pollen tube when 3H-methyl methionine is used. A finding that was unexpected was that pectin synthesized in pollen tubes can be isolated on a methylated albumin on kieselguhr column just before the tRNA begins to elute with salt.
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