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Optical mapping as a routine tool for bacterial genome sequence finishing

2007; BioMed Central; Volume: 8; Issue: 1 Linguagem: Inglês

10.1186/1471-2164-8-321

1471-2164

Phil Latreille, Stacie Norton, Barry S. Goldman, John Henkhaus, Nancy Miller, W. Brad Barbazuk, Helge B. Bode, Creg Darby, Zijin Du, Steve Forst, Sophie Gaudriault, Brad Goodner, Heidi Goodrich‐Blair, Steven Slater,

Insect symbiosis and bacterial influences

Abstract Background In sequencing the genomes of two Xenorhabdus species, we encountered a large number of sequence repeats and assembly anomalies that stalled finishing efforts. This included a stretch of about 12 Kb that is over 99.9% identical between the plasmid and chromosome of X. nematophila . Results Whole genome restriction maps of the sequenced strains were produced through optical mapping technology. These maps allowed rapid resolution of sequence assembly problems, permitted closing of the genome, and allowed correction of a large inversion in a genome assembly that we had considered finished. Conclusion Our experience suggests that routine use of optical mapping in bacterial genome sequence finishing is warranted. When combined with data produced through 454 sequencing, an optical map can rapidly and inexpensively generate an ordered and oriented set of contigs to produce a nearly complete genome sequence assembly.