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Isolation and characterization of 27 polymorphic microsatellite loci for the common snook, Centropomus undecimalis

2005; Wiley; Volume: 5; Issue: 4 Linguagem: Inglês

10.1111/j.1471-8286.2005.01118.x

1471-8286

Seifu Seyoum, Michael D. Tringali, Jamie G. Sullivan,

Fish Ecology and Management Studies

Abstract Here we describe 32 di‐, tri‐ and tetranucleotide microsatellite loci isolated by PIMA, a polymerase chain reaction (PCR)‐based procedure, for the common snook ( Centropomus undecimalis ). Five loci were monomorphic, and the remaining loci averaged 6.7 alleles per locus in a sample of 45 common snook. For polymorphic loci, expected heterozygosities ranged from 0.02 to 0.91 (mean = 0.538). Significant departures from Hardy–Weinberg equilibrium expectations occurred in two loci. Exact tests for genotype disequilibrium gave evidence for linkage at one pair of loci. Many cross‐species primer assays yielded PCR fragments of the expected size for 11 species of Centropomus and two species of the confamilial genus Lates .