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Artigo Revisado por pares
BIBTEX RIS

Improved multiplex PCR method for the rapid detection of β-lactamase genes in Escherichia coli of animal origin

2006; Elsevier BV; Volume: 56; Issue: 1 Linguagem: Inglês

10.1016/j.diagmicrobio.2006.03.005

ISSN

1879-0070

Autores

Constança Pomba, Nuno Mendonça, Marta Costa, Deolinda Louro, Bruno Baptista, Mónica S. Ventura Ferreira, José Duarte Correia, Manuela Caniça,

Tópico(s)

Antibiotics Pharmacokinetics and Efficacy

Resumo

We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).

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