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Cellular Enrichment with Polyunsaturated Fatty Acids Induces an Oxidative Stress and Activates the Transcription Factors AP1 and NFκB

1999; Elsevier BV; Volume: 265; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1999.1644

1090-2104

Cécile Mazière, Marie-Alix Conte, James Degonville, Dany Ali, Jean‐Claude Mazière,

Genomics, phytochemicals, and oxidative stress

A 48-h incubation of cultured human fibroblasts with 5 × 10−5 M oleic acid or polyunsaturated fatty acids (PUFA) from the (n-6) (linoleic, γ-linolenic and arachidonic acids) or (n-3) (α-linolenic and eicosapentaenoic acids) series resulted in an enrichment of the cells with the introduced fatty acid. Cell enrichment with PUFA initiated a rise in the intracellular level of reactive oxygen species (ROS) and lipid peroxidation products (thiobarbituric reactive substances TBARS). Simultaneously, cell enrichment with all the studied PUFA induced an increase in AP1 and NFκB binding activity measured by electrophoretic mobility shift assay, whereas no significant effect was observed with the monounsaturated oleic acid. Furthermore, the antioxidants vitamin E (α-tocopherol) and N-acetyl cysteine prevented both the arachidonic acid-induced increase in intracellular ROS and TBARS, and the activation of AP1 and NFκB. These results indicate that the accumulation of PUFA from (n-6) and (n-3) series elicited an intracellular oxidative stress, resulting in the activation of oxidative stress-responsive transcription factors such as AP1 and NFκB.