HPLC separation of 32P-postlabelled benzo[b]fluoranthene-DNA adducts
1992; Elsevier BV; Volume: 65; Issue: 2 Linguagem: Inglês
10.1016/0304-3835(92)90161-n
ISSN1872-7980
AutoresWolfgang Pfau, Nicola Hughes, P.L. Grover, David H. Phillips,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoAnalysis using 32P-postlabelling and a recently developed HPLC method resolved the adduct formed by reaction of the benzo[b]fluoranthene (BbF) anti-bay-region diol-epoxide with DNA from the more polar major adduct produced by the hydrocarbon in three different biological systems. In each case, the adduct formed from the anti-bay-region diol-epoxide constituted only a minor proportion of the total DNA modification. Comparisons of the DNA adducts formed from the hydrocarbon with those formed in microsomal incubations from the putative metabolites BbF-9,10-diol, anti-BbF-9,10-diol-11,12-oxide and the 5,9,10- and 6,9,10-BbF-triols indicate that the predominant pathway for BbF activation in skin probably involves a bayregion triol-epoxide possessing a phenolic OH-group on the peninsula ring.
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