A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor δ-chain-encoding gene
1991; Elsevier BV; Volume: 105; Issue: 2 Linguagem: Inglês
10.1016/0378-1119(91)90161-4
ISSN1879-0038
AutoresNathalie van der Lugt, Ets Robanus Maandag, Hein te Riele, Peter W. Laird, Anton Berns,
Tópico(s)CAR-T cell therapy research
ResumoWe have constructed a hypoxanthine phosphoribosyl transferase-selectable marker (hprt) under the control of the phosphoglycerate kinase (pgk) promoter. This construct permits cell growth in hypoxanthine/aminopterin/thymidine media and confers 6-thioguanine sensitivity upon mouse Hprt− embryonic stern cells, allowing either positive or negative selection in gene-targeting experiments. We have successfully targeted the gene encoding the T-cell receptor δ-chain using the pgkr.hprt fusion for counterselection.
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