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Platelets prevent acute liver damage after extended hepatectomy in pigs

2010; Wiley; Volume: 17; Issue: 6 Linguagem: Inglês

10.1007/s00534-010-0276-2

1868-6982

Katsuji Hisakura, Soichiro Murata, Kiyoshi Fukunaga, Andriy Myronovych, Sosuke Tadano, Takuya Kawasaki, Keisuke Kohno, Osamu Ikeda, Sugiru Pak, Naoya Ikeda, Yoritaka Nakano, Ryota Matsuo, Kenjiro Konno, Eiji Kobayashi, Toshiyuki Saito, Hiroshi Yasue, Nobuhiro Ohkohchi,

Pancreatic function and diabetes

Abstract Background/Purpose Platelets develop tissue repair and promote liver regeneration. We investigated whether platelets prevented acute liver damage after extended hepatectomy in pigs. Methods Thrombocytosis was induced by the following two methods; afterwards 80% hepatectomy was performed in pigs. In the first method, the pigs received administration of thrombopoietin [TPO (+) group], and they were compared with a control group [TPO (−) group]. In the second method, the pigs received a splenectomy [Sp (+) group], and theywere compared with another control group [Sp (−) group]. Platelet counts, biochemical examination of blood, and histopathological findings of the residual liver were examined. Results Serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin (T‐Bil) levels were significantly decreased in the thrombocytotic groups compared with the control groups in the early period after hepatectomy. In the histopathological findings, hemorrhagic necrosis with a bile plug was observed in the control groups, but this phenomenon was not observed in the thrombocytotic groups. On transmission electron microscopy, the sinusoidal endothelial lining was destroyed and detached into the sinusoidal space with enlargement of Disse's spaces in the thrombocytotic groups, but these findings were not observed in the control groups. Conclusion An increased number of platelets prevents acute liver damage after extended hepatectomy.