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High throughput detection of antibody self-interaction by bio-layer interferometry

2013; Landes Bioscience; Volume: 5; Issue: 6 Linguagem: Inglês

10.4161/mabs.26186

1942-0870

Tingwan Sun, Felicia Reid, Yuqi Liu, Yuan Cao, Patricia A. Estep, Claire Nauman, Yingda Xu,

Protein purification and stability

Self-interaction of an antibody may lead to aggregation, low solubility or high viscosity. Rapid identification of highly developable leads remains challenging, even though progress has been made with the introduction of techniques such as self-interaction chromatography (SIC) and cross-interaction chromatography (CIC). Here, we report a high throughput method to detect antibody clone self-interaction (CSI) using bio-layer interferometry (BLI) technology. Antibodies with strong self-interaction responses in the CSI-BLI assay also show delayed retention times in SIC and CIC. This method allows hundreds of candidates to be screened in a matter of hours with minimal material consumption.