Artigo Acesso aberto Revisado por pares

TARGETING OF A PLASMA CELL LINE WITH A CONJUGATE CONTAINING XANTHINE OXIDASE AND THE MONOCLONAL ANTIBODY 62B1

1989; Wolters Kluwer; Volume: 48; Issue: 1 Linguagem: Inglês

10.1097/00007890-198907000-00028

ISSN

1534-6080

Autores

Pier Luigi Tazzari, Maria Giulia Battelli, Ada Abbondanza, Angelo Dinota, Simonetta Rizzi, Marco Gobbi, Fiorenzo Stirpe,

Tópico(s)

Hematopoietic Stem Cell Transplantation

Resumo

We report on the preparation of an antibody-conjugated enzyme consisting of xanthine oxidase, a free-radical-producing enzyme, linked to the 62B1 monoclonal antibody, which recognizes the last steps of differentiation of B cell lineage (plasma cell and hairy cells). The conjugate specifically kills target cells, retaining both enzymic and immunological properties, without any damage to normal myeloid clonogenic efficiency. The model is suitable for ex vivo bone marrow purging in multiple myeloma patients.

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