SUPPLEMENTATION OF IN-VITRO FERTILISATION CULTURE MEDIUM WITH PLATELET ACTIVATING FACTOR
1989; Elsevier BV; Volume: 334; Issue: 8666 Linguagem: Inglês
10.1016/s0140-6736(89)90831-3
ISSN1474-547X
AutoresC. O’Neill, M. Collier, Alaina J. Ammit, John P. Ryan, D. M. Saunders, Ian Pike,
Tópico(s)Periodontal Regeneration and Treatments
ResumoThe supplementation of culture medium with platelet activating factor (PAF) on the subsequent implantation and pregnancy potential of pre-embryos produced by in-vitro fertilisation was studied. Pre-embryo culture medium was supplemented with 0 (control), 0.186, 0.93, or 1.49 mumol/1 PAF. Pre-embryos were transferred to PAF-containing medium 15-17 h after insemination (ie, just before syngamy) for 24 h and then transferred to the uterus. For 185 women receiving control pre-embryos, the pregnancy rate (positive beta human chorionic gonadotropin per oocyte retrieval) was 10.2%, while 166 women who received PAF treated pre-embryos (all concentrations combined) achieved a pregnancy rate of 17.5%. This difference was significant. The pregnancy rates per pre-embryo transferred were 6.1% and 9.4% for the control and PAF groups, respectively. The percentage of positive pregnancy tests that resulted in a viable pregnancy (presence of fetal heart at 8 weeks) was 78.9% in the controls and 75.9% in the PAF group. There was no difference in the average number of embryos transferred in either group. The increase in the pregnancy rate after a short exposure of pre-embryos to PAF in vitro suggests that PAF mediates pre-embryo development.
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