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Leishmaniasis in Brazil. XXII: Characterization of Leishmania from man, dogs and the sandfly Lutzomyia longipalpis (Lutz & Neiva 1912) isolated during an outbreak of visceral leishmaniasis in Santarém, Pará State

1986; Oxford University Press; Volume: 80; Issue: 1 Linguagem: Inglês

10.1016/0035-9203(86)90214-2

1878-3503

R.R. Braga, R. Lainson, J. J. Shaw, L. Ryan, Fernando Tobias Sílveira,

Research on Leishmaniasis Studies

During epidemiological studies on an outbreak of visceral leishmaniasis in Santarém, Pará State, north Brazil, isolates of Leishmania from two children, three dogs and six naturally infected specimens of the sandfly Lutzomyia longipalpis were compared, biochemically, by starch-gel enzyme electrophoresis. They have proved to be indistinguishable from each other, and from a reference strain of Leishmania chagasi Cunha & Chagas 1937 from a case of human visceral leishmaniasis from Bahia State, north-east Brazil, on their enzyme profiles for ASAT, ALAT, PGM, GPI, MDH and MPI. Lu. longipalpis is the principal, and possibly the only vector to man in the Amazon Region of Brazil. Cepas de Leishmania isoladas do homem, cães e de flebotomíneos (Lutzomyia longipalpis) foram comparadas com base na sua eletroforese isoenzimatica (ASAT, ALAT, PGM, GPI, MDH, e MPI) procedida em gel de amido. Os perfis eletroforeticos obtidos foram indistinguiveis entre si, assim como não diferem, nesse particular, de amostra padrão de Leishmania chagasi oriunda de um caso de leishmaniose visceral humana procedente do Estado da Bahia, nordeste do Brasil.