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Artigo Revisado por pares
BIBTEX RIS

Purification of copper-zinc-superoxide dismutase and catalase from human erythrocytes by copper-chelate affinity chromatography

1986; Elsevier BV; Volume: 370; Linguagem: Inglês

10.1016/s0021-9673(00)94720-4

ISSN

1873-3778

Autores

Misako Miyata-Asano, Keizo Ito, Hisami Ikeda, Sadayoshi Sekiguchi, K Arai, Naoyuki Taniguchi,

Tópico(s)

Protein Interaction Studies and Fluorescence Analysis

Resumo

A relatively simple and reproducible procedure involving copper-chelate affinity chromatography for the isolation of copper-zinc-superoxide dismutase (E.C. 1.15.1.1) and catalase (E.C. 1.11.1.6) from human erythrocytes has been developed. Using this method, the two enzymes were easily and highly purified and appeared to be homogeneous as judged by polyacrylamide gel electrophoresis.

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