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Dual mode monitoring probe for mitochondrial viscosity in single cell

2013; Elsevier BV; Volume: 190; Linguagem: Inglês

10.1016/j.snb.2013.09.062

1873-3077

Na Jiang, Jiangli Fan, Si Zhang, Tong Wu, Jingyun Wang, Pan Gao, Junle Qu, Fan Zhou, Xiaojun Peng,

Porphyrin and Phthalocyanine Chemistry

Viscosity strongly influences intracellular transportation of mass and signal, and although bulk viscosity can be well measured with macroscopic samples quantities, microscale measurements in live cells still remain a challenge. By introducing a CHO group into the meso-position of the pentamethine chain, a thiazole-Cy5 dye based two-photon fluorescence probe, Mito-V, was developed. It exhibited a selective response to solution viscosity by fluorescence ratio and lifetime methods. More importantly, Mito-V can localize in mitochondria and quantitatively image its viscosity changes by the dual fluorescence mode during the apoptosis.