Presentation of Peptide Antigens as Albumin Conjugates for Use in Detection of Serum Antibodies by Enzyme-Linked Immunosorbent Assay
1996; American Chemical Society; Volume: 7; Issue: 3 Linguagem: Inglês
10.1021/bc960018s
ISSN1520-4812
AutoresZhiguang Yu, John M. Carter, Shaei‐Yun Huang, Henry Lackland, Leonard H. Sigal, Stanley J. Stein,
Tópico(s)Viral Infections and Vectors
ResumoThe use of linear peptides as antigens for detection of serum antibodies has been studied using a sequence of the Borrelia burgdorferi protein, flagellin, and Lyme disease sera as a model. It was found that a novel presentation of the peptide as a hapten on the carrier protein, bovine serum albumin, in the enzyme-linked immunosorbent assay format can be successfully applied to distinguish between Lyme disease and control sera.
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