Expression of α,βA and βB subunits of inhibin or activin and follistatin in rat pancreatic islets
1993; Wiley; Volume: 319; Issue: 3 Linguagem: Inglês
10.1016/0014-5793(93)80549-a
ISSN1873-3468
AutoresKenji Ogawa, Keiko Abe, Nobuyuki Kurosawa, Masamichi Kurohmaru, Hiromu Sugino, Michio Takahashi, Yoshihiro Hayashi,
Tópico(s)Caveolin-1 and cellular processes
ResumoWe first detected the mRNA expression of follistatin and three subunits of inhibin/activin in rat pancreatic islets by reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemistry using anti-follistatin serum (against residues 123-134) revealed that follistatin was localized only in insulin-producing B cells. Although the beta A subunit was detectable in the islets, the immunostainable cell types were completely different with two beta A antisera, i.e. anti-beta A (1-10)-Tyr stained B cells, while anti-beta A (87-99) stained glucagon-producing A cells. This inconsistent immunoreactivity was probably related to follistatin binding to beta subunits of inhibin/activin. This study indicates that follistatin and inhibin/activin in the islet serve as paracrine or autocrine modulators in the endocrine pancreas.
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