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Intermittent Fasting Promotes Bacterial Clearance and Intestinal I g A Production in S almonella typhimurium ‐Infected Mice

2014; Wiley; Volume: 79; Issue: 5 Linguagem: Inglês

10.1111/sji.12163

1365-3083

Marycarmen Godínez‐Victoria, Rafael Campos‐Rodríguez, Víctor Rivera‐Aguilar, Eleazar Lara‐Padilla, Judith Pacheco‐Yépez, Rosa Adriana Jarillo‐Luna, María Elisa Drago-Serrano,

Diet and metabolism studies

Abstract The impact of intermittent fasting versus ad libitum feeding during S almonella typhimurium infection was evaluated in terms of duodenum I g A levels, bacterial clearance and intestinal and extra‐intestinal infection susceptibility. Mice that were intermittently fasted for 12 weeks or fed ad libitum were infected with S . typhimurium and assessed at 7 and 14 days post‐infection. Next, we evaluated bacterial load in the faeces, P eyer's patches, spleen and liver by plate counting, as well as total and specific intestinal I g A and plasmatic corticosterone levels (by immunoenzymatic assay) and lamina propria I g A levels in plasma cells (by cytofluorometry). P olymeric immunoglobulin receptor, α ‐ and J ‐chains, P ax‐5 factor, pro‐inflammatory cytokine (tumour necrosis factor‐ α and interferon‐ γ ) and anti‐inflammatory cytokine (transforming growth factor‐ β ) mRNA levels were assessed in mucosal and liver samples (by real‐time PCR ). Compared with the infected ad libitum mice, the intermittently fasted infected animals had (1) lower intestinal and systemic bacterial loads; (2) higher S Ig A and I g A plasma cell levels; (3) higher mRNA expression of most intestinal parameters; and (4) increased or decreased corticosterone levels on day 7 and 14 post‐infection, respectively. No contribution of liver IgA was observed at the intestinal level. Apparently, the changes following metabolic stress induced by intermittent fasting during food deprivation days increased the resistance to S . typhimurium infection by triggering intestinal I g A production and presumably, pathogen elimination by phagocytic inflammatory cells.