Produção Nacional
Revisado por pares
Shrimp Waste Extract and Astaxanthin: Rat Alveolar Macrophage, Oxidative Stress and Inflammation
2012; Wiley; Volume: 77; Issue: 7 Linguagem: Inglês
10.1111/j.1750-3841.2012.02762.x
ISSN1750-3841
AutoresSuzan Diniz Santos, Thiago Barbosa Cahú, Guilherme Oliveira Firmino, Célia C M M B de Castro, Luiz B. Carvalho, Ranilson de Souza Bezerra, José Luiz Lima Filho,
Tópico(s)Aquaculture Nutrition and Growth
ResumoAstaxanthin is a carotenoid known to have antioxidant and antiinflammatory properties. This study examined if shrimp astaxanthin modulates the production of superoxide (O(-)(2)), nitric oxide (NO), and tumor necrosis factor-α (TNF-α) in rat alveolar macrophages. The oxidative effect was induced by phorbol myristate acetate and lipopolysacharide. The treatment was compared with superoxide dismutase, butylated hydroxytoluene, commercial astaxanthin, N-nitric-L-arginine methyl ester and L- canavanine, all administered as a 43.5-μg/mL dose in the presence of 1% EtOH/0.5% DMSO. All treatments maintained cell viability, as observed in the MTT assay, and shrimp extract increased the viable alveolar macrophages to 168%. Shrimp extract and commercial astaxanthin showed a suppressive effect on the generation of both free radicals O(-)(2) and NO, while purified shrimp astaxanthin was specific to NO. TNF-α secretion was correlated with NO production. However, in this correlation, the shrimp extract completely inhibited TNF-α. In the light of these findings, the antioxidant action demonstrated in this study suggests that the shrimp extract could be considered as a promising source of bioactive substances with antioxidant and anti-inflammatory activity.The hydrolysis process of shrimp waste generates bioactive products that add economic value to shrimp processing, mainly because they may have applications in nutraceutical and animal feed industry.
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