Expression of Ti-plasmid DNA in E. coli: Comparison of homologous fragments cloned from Ti plasmids of Agrobacterium strains C58 and Ach5
1980; Elsevier BV; Volume: 4; Issue: 2 Linguagem: Inglês
10.1016/0147-619x(80)90009-8
ISSN1095-9890
AutoresSilvia Schweitzer, Dietmar Blohm, Klaus Geider,
Tópico(s)Enzyme Production and Characterization
ResumoFragments of two Ti plasmids from Agrobacterium tumefaciens were cloned and studied for cross-hybridization. Homologous fragments were further investigated for areas of strong homology and mapped with various restriction enzymes. The fragments were inserted in two directions and the recombinant plasmid was brought into E. coli strains producing minicells. About five proteins were found to be coded by those fragments of the Ti plasmids.
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