Cell Cycle Dependence of Chloride Permeability in Normal and Cystic Fibrosis Lymphocytes
1990; American Association for the Advancement of Science; Volume: 248; Issue: 4961 Linguagem: Inglês
10.1126/science.2162561
ISSN1095-9203
AutoresJames K. Bubien, Kevin L. Kirk, Thomas A. Rado, Raymond A. Frizzell,
Tópico(s)Neonatal Respiratory Health Research
ResumoCystic fibrosis (CF) is a genetic disease characterized by abnormal regulation of epithelial cell chloride channels. Nonepithelial cells, including lymphocytes and fibroblasts, may exhibit a similar defect. Two independent techniques were used to assess the macroscopic chloride permeability ( P Cl ) of freshly isolated B lymphocytes and of B and T lymphocyte cell lines. Values for P Cl increased specifically during the G 1 phase of the cell cycle and could be further enhanced by increasing intracellular adenosine 3′,5′-monophosphate (cAMP) or calcium. In lymphocytes from CF patients, regulation of P Cl during the cell cycle and by second messengers was absent. Characterization of the cell cycle-dependent expression of the chloride permeability defect in lymphocytes from CF patients increases the utility of these cells in the analysis of the functional consequences of mutations in the CF gene.
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