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Population structure of algicidal marine bacteria targeting the red tide forming alga Heterosigma akashiwo (Raphidophyceae), determined by restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA genes

1998; Inter-Research; Volume: 170; Linguagem: Inglês

10.3354/meps170033

1616-1599

Ikuo Yoshinaga, Kim Mc, N Katanozaka, Ichiro Imai, Aya Uchida, Yukimasa Ishida,

Marine Biology and Ecology Research

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 170:33-44 (1998) - doi:10.3354/meps170033 Population structure of algicidal marine bacteria targeting the red tide forming alga Heterosigma akashiwo (Raphidophyceae), determined by restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA genes Ikuo Yoshinaga1,*, Mu-Chan Kim1, Noriaki Katanozaka1, Ichiro Imai1, Aritsune Uchida1, Yuzaburo Ishida2 1Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan 2Faculty of Engineering, Fukuyama University, Fukuyama, Hiroshima 7290292, Japan *E-mail: iyoshina@kais.kyoto-u.ac.jp ABSTRACT: A total of 233 marine bacterial strains which killed a noxious marine microalga, Heterosigma akashiwo, were isolated from Hiroshima Bay, the Seto Inland Sea, Japan, during blooms of H. akashiwo in 1994 and 1995. Population structure and genetic diversity of the H. akashiwo-killing bacteria (HAKB) were analyzed by means of restriction fragment length polymorphism (RFLP) and partial sequences of 16S ribosomal RNA genes (16S rDNA) PCR (polymerase chain reaction) amplified from HAKB strains. The RFLPs were generated by separate digestion with 5 restriction enzymes, Eco RI, Rsa I, Mbo I, Bst UI and Hha I. Seventeen ribotypes were observed among 85 strains of HAKB isolated in 1994. Bacterial strains of 3 ribotypes, 2B, 2C and 2D, were dominant in the HAKB populations during the termination period of the H. akashiwo bloom concurrent with the increase in the number of HAKB. Partial sequences, almost 500 bp of nucleotides, and RFLP patterns of 16S rDNA from some HAKB strains revealed that the HAKB of 2B, 2C and 2D ribotypes are closely related to the γ -proteobacteria group. The HAKB strains belonging to 2C and 2D were repeatedly isolated from seawater collected at the end of a H. akashiwo bloom in 1995. These results suggest that 3 species of HAKB may play a role in the rapid termination of the H. akashiwo bloom in Hiroshima Bay. KEY WORDS: Algicidal bacteria · Marine bacteria · 16S rRNA · Restriction fragment length polymorphism · Heterosigma akashiwo · Marine phytoplankton · Bloom Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 170. Publication date: September 03, 1998 Print ISSN:0171-8630; Online ISSN:1616-1599 Copyright © 1998 Inter-Research.