Histochemische charakterisierung der hämolymphe-proteine aus kokonstadien von Formica-arten nach polyacrylamidelektrophorese
1976; Elsevier BV; Volume: 6; Issue: 4 Linguagem: Inglês
10.1016/0020-1790(76)90042-1
ISSN1879-2928
AutoresGerhard Schmidt, Walter Schwankl, Barbara Wirth,
Tópico(s)Insect and Arachnid Ecology and Behavior
ResumoThe haemolymph proteins of Formica rufa (polygynous), F. pratensis, and F. polyctena cocoon stages separated on 7% polyacrylamide gels stain with aniline blue black, Schiffs reagent, methylene blue, Sudan Black B, iodine, Feulgen, and methylgreen-pyronin method. Complex proteins were demonstrated as the slow-running fractions and in the front band. On these proteins DNA, RNA, lipids, and glycides were bound during metamorphosis. Separation with sodium dodecyl sulfate (SDS) showed selective splitting of the slow-running fractions. Long term separation or separation in less dense gels showed that the fractions were basically similar with the exception of the slow-running portions.
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