Portal de Periódicos da CAPES

Localization of 3 H‐estradiol in the reproductive organs of male and female baboons

1982; Wiley; Volume: 172; Issue: 2 Linguagem: Inglês

10.1002/jmor.1051720203

1097-4687

Frank J. Weaker, Peter J. Sheridan,

Estrogen and related hormone effects

Abstract The uptake and retention of radiolabeled estradiol by both the male and female reproductive organs were examined in the baboon. Two male and two female baboons were injected intracardially with 1 μg/kg body weight of 3 H‐estradiol and two animals, one male and one female, were injected with both labeled and 100 μg/kg body weight of unlabeled estradiol. One and a half hours after the injections, the animals were sacrificed and the uterus, cervix, vagina, oviduct, seminal vesicles, and prostate gland were removed and processed for autoradiography. The stratified squamous epithelia of the cervix and vagina demonstrated a light uptake of the label in the germinative, but not in the superficial cell layers. The columnar cells lining the oviduct and uterine glands were labeled, whereas the luminal epithelium of the uterus and the glandular epithelia of the seminal vesicles and prostate gland did not sequester the tritiated steroid. The interstitial cells of all the organs studied demonstrated a moderate to heavy uptake of the radioactivity, whereas the smooth muscle cells were lightly labeled except in the vagina, in which these cells displayed a moderate number of silver grains.