Reactions of partially oxidised hemoglobin solutions I. Separation of intermediate compounds by CM-sephadex chromatography
1968; Elsevier BV; Volume: 160; Issue: 1 Linguagem: Inglês
10.1016/0005-2795(68)90058-5
ISSN1878-1454
Autores Tópico(s)Neonatal Health and Biochemistry
ResumoThe separation of hemoglobin derivatives containing two iron atoms in the ferric state is described. Using a sheep hemoglobin of the B type, it was found that a pH-gradient elution of partially oxidised oxy- or carboxyhemoglobin from CM-Sephadex produced three fractions which, in order of elution, corresponded to unoxidised, half-oxidised and wholly oxidised pigment. The same result followed if hemoglobin derivatives were mixed with methemoglobin solutions and stored for several days.
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