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Catalyser-21TM, a mineral water derived from leaf soil, inhibits tumor cell invasion and angiogenesis

2007; Springer Science+Business Media; Volume: 55; Issue: 2-3 Linguagem: Inglês

10.1007/s10616-007-9073-4

1573-0778

Jun Ye, Yuping Li, Takeki Hamasaki, Noboru Nakamichi, Takeshi Kawahara, Kazuhiro Osada, Kiichiro Teruya, Yuko Kato, Kazuko Toh, Masumi Abe, Yoshinori Katakura, Katsumi Noguchi, Sanetaka Shirahata,

Cancer Cells and Metastasis

Catalyser-21TM is a mineral water derived from natural leaf soil containing various organic and inorganic substances. Previous reports suggested a possibility that Catalyser-21TM has antioxidative potential and could inhibit angiogenesis and cancer cell invasiveness. Angiogenesis is a prerequisite for cancer cells to spread to surrounding tissues. Vascular endothelial growth factor (VEGF) is a major angiogenic factor in the formation of blood capillaries by cancer cells to supply nutrients and oxygen for their sustained growth. Matrix metalloproteinase-2 (MMP-2) is another key enzyme for cancer cell metastasis. To assess the anti-angiogenic activity of Catalyser-21TM, we first examined cell viability using a human cervical cancer cell line, HeLa, and a fibrosarcoma cell line, HT1080. The results showed that Catalyser-21TM decreased the viability of both cell types in a dose-dependent manner. Flow cytometric analysis proved that Catalyser-21TM scavenges intracellular H2O2 in both cell types. RT-PCR demonstrated that both VEGF and MMP-2 gene transcription was suppressed after Catalyser-21TM treatment. Both Matrigel and tubule formation experiments showed an effect of Catalyser-21TM. These results suggest that Catalyser-21TM has potential as an anti-tumor agent.