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Receptors for IgG2b on cells of the mouse metrial gland

1989; Elsevier BV; Volume: 16; Issue: 3 Linguagem: Inglês

10.1016/0165-0378(89)90054-5

1872-7603

N.M. Daki, Ian Stewart, A. E. Wild,

Protein purification and stability

Single cells prepared from metrial glands of mice killed at days 10, 13 and 17 of pregnancy were assayed for the expression of Fcγ receptors in a standard rosetting assay using sheep red blood cells sensitised with a mouse monoclonal IgG2b antibody. Rosettes, indicating Fcγ receptors, were found on both granulated metrial gland (GMG) cells and non-GMG cells, comprising mainly stromal cells, from each stage of pregnancy. Some animals were given an intravenous injection of horseradish peroxidase 2 h before they were killed in order to identify endocytic cells. No GMG cells were found to have endocytosed the horseradish peroxidase. Non-GMG cells which showed endocytic activity all expressed Fcγ receptors but these receptors were also found on some of the non-GMG cells which had not exhibited endocytosis. The finding of Fcγ receptors on GMG cells provides further evidence that these cells may be related to NK cells.